11th Annual Meeting of Proteomics Society, India, 2019 and International Conference on Proteomics for System Integrated Bio-Omics, One Health and Food Safety
2nd- 4th Dec 2019,
ICAR-NDRI, Karnal, INDIA

Metabolomics tools for addressing Biological Problems

Metabolomics is the large-scale study of small molecules, commonly known as metabolites, within cells, biofluids, tissues or organisms. Collectively, these small molecules and their interactions within a biological system are known as the metabolome.

Just as genomics is the study of DNA and genetic information within a cell, and transcriptomics is the study of RNA and differences in mRNA expression; metabolomics is the study of substrates and products of metabolism, which are influenced by both genetic and environmental factors.

Metabolomics is a powerful approach because metabolites and their concentrations, unlike other "omics" measures, directly reflect the underlying biochemical activity and state of cells / tissues. Thus metabolomics best represents the molecular phenotype.

The ICPBHF-2019 Metabolomics day/workshop will consist of a variety of plenary and parallel sessions, aimed to discuss the recent highlights, implementation of methodologies in statistics, missing data, study design role play, of important knowledge achieved through the application of metabolomics data in the field. Finally, speculate about the future directions of metabolomics approaches.

As a part of this intensive training, each participant will get hands-on training to understand the mass spectrometry-derived metabolomics data analysis and also the collaboration of metabolomics data to other proteomics and genomics data.

Agenda for the PSI’19 Metabolomics Day/workshop

Experimental (Lectures)

  • Introduction: from Metabolism to Metabolomics (technology and applications)
  • Experimental design for a metabolomics experiment
  • The importance of sample preparation, metabolite extraction methods and storage
  • LC-MS workflow (separation and detection techniques for metabolomic analysis)
  • Overview of Data analysis and Metabolite annotations

Experimental (Hands-on)

  • Workflows for Targeted metabolomics and Untargeted metabolomics
  • Quality control system

Data analysis (Hands-on with LC-MS raw data)

  • Evaluation of raw data quality with Quality control system
  • Data processing workflow: from feature detection to annotation and quantification
  • Statistical analysis and visualization of the data
  • Metabolite identification and spectral libraries
  • Metabolite pathway analysis

Introduction to advance metabolomics technologies:

  • 13C-tracer based metabolomics
  • Imaging mass spectrometry for spatial metabolomics
  • Spectral libraries for metabolite identifications
  • Short survey of participants at the begning and feedback at the end of the workshop